王冰清¹，徐 浪¹，胡力飞¹，刘 洋²，张 帆¹，杨 强²，殷 涛^1*

（1.劲牌持正堂药业有限公司，湖北省中药配方颗粒工程研究中心，黄石 435000；

2.劲牌有限公司，黄石 435100）

摘 要：该文建立了生姜药材特征图谱及以单一内标物计算多种成分相对含量的“质-量”双标的质量评价方法。具体方法为：采用超高效液相色谱（UPLC）和四极杆-飞行时间质谱（Q-TOF MS）技术建立了生姜药材特征图谱，标定了其共有特征峰并明确其中的化学成分，通过比对供试品药材和对照药材的相似度，即可明确药材的真伪，即为“质”；对内标成分“6-姜辣素”进行准确的定量，同时通过内标成分计算各特征峰的相对含量，再根据各特征峰的相对含量下限评价生姜药材的优劣，即为“量”。结果表明，通过该方法建立的特征图谱和内标成分含量测定方法均符合方法学要求；经过分析，特征图谱中发现了10个共同的特征峰，并且确定了其中的4种化学成分；此外，不同产地生姜药材与对照药材的相似度均超过了0.900，同时也设定了各特征峰的相对含量下限。该方法不依赖多个对照品且能同时在质和量两方面做到全面、快速、准确地对药材的真伪优劣进行评价，为全面控制生姜药材的质量提供了参考依据。

关键词：生姜；超高效液相色谱；特征图谱；内标物质；质量评价；6-姜辣素

中图分类号：TS264.3  文献标识码：A  文章编号：1674-506X（2026）01-0122-0009

Research on the Quality Evaluation Method of Ginger Medicinal Materials Based on UPLC“Quality-Quantity”Double Standards

WANG Bingqing¹，XU Lang¹，HU Lifei¹，LIU Yang²，ZHANG Fan¹，YANG Qiang²，YIN Tao^1*

（1.Jinpai Chizhengtang Pharmaceutical Co.，Ltd.，Hubei Provincial Engineering Research Center for Traditional Chinese Medicine Formula Granules，Huangshi 435000，China

2.Jinpai Co.，Ltd.，Huangshi 435100，China）

Abstract：In this article，the characteristic chromatogram of ginger medicinal material and the quality evaluation method of“Quality-Quantity”double standards with single internal standard that calculates the relative content of various components were established. The specific method：ultra performance liquid chromatography （UPLC）and quadrupole-time-of-flight mass spectrometry （Q-TOF MS） were used to establish the characteristic chromatogram of ginger medicinal material， and the common characteristic peaks were calibrated and its chemical components were identified. By comparing the similarity between the test medicinal materials and the reference medicinal materials，the authenticity of the medicinal materials can be determined，which is called “Quality”；the internal standard component“6-gingerol”was accurately quantified，simultaneously，the relative content of each characteristic peak was calculated through the internal standard component，the quality of ginger medicinal materials was then evaluated based on the lower limits of the relative content of each characteristic peak， which is called “Quantity”. Results showed the characteristic chromatogram and internal standard component content determination method established by this method all met the methodological requirements；After analysis，a total of 10 common characteristic peaks were found in the characteristic chromatogram，with 4 chemical components identified；Moreover，the similarity between ginger medicinal materials from different origins and the reference medicinal materials exceeded all 0.900，and the lower limits of relative content of each characteristic peak were also established. This method does not rely on multiple reference standards and can comprehensively，rapidly，and accurately evaluate the authenticity and quality of medicinal materials in both qualitative and quantitative aspects. It provides a reference basis for comprehensively controlling the quality of gingermedicinal materials.

Keywords：ginger；UPLC；characteristic chromatogram；internal standard substance；quality evaluation；6-gingerol

doi：10.3969/j.issn.1674-506X.2026.01-017