徐懿乔¹，宋娟娟^2,3*，周彬^2,3，冯洁¹，李伟^2,3，王晨 ^2,3，王忠稳^2,3，曹菁^2,3，郝江伟^2,3，黄盼^2,3，傅雅丽⁴

（1.杭州环特生物科技股份有限公司,杭州 310051; 2.劲牌持正堂药业有限公司,黄石 435000;

3.中药保健食品质量与安全湖北省重点实验室（劲牌有限公司）,大冶 435100;

4.黄石市食品药品检验检测中心,黄石 435000）

摘 要：研究藿香紫苏饮对由酒石酸长春瑞滨所诱导的免疫力低下斑马鱼的免疫功能的调节作用。通过给斑马鱼静脉注射0.2 mg/mL酒石酸长春瑞滨诱导建立免疫力低下模型，将斑马鱼随机分为正常对照组、模型对照组、藿香紫苏饮低剂量组（500μg/mL）、藿香紫苏饮中剂量组（1000μg/mL）、藿香紫苏饮高剂量组（2000μg/mL），每组30尾。使用荧光显微镜观察并分析各组斑马鱼巨噬细胞吞噬功能及T细胞减少的改善效果。结果表明，藿香紫苏饮高剂量组（2000μg/mL）斑马鱼体内剩余荧光微球数量显著低于模型对照组（P＜0.05）；藿香紫苏饮低、中、高剂量组斑马鱼T细胞荧光强度显著高于模型对照组（P＜0.01）。藿香紫苏饮具有促进斑马鱼巨噬细胞吞噬、改善T细胞减少的功效。

关键词：藿香紫苏饮；斑马鱼；巨噬细胞；T细胞；吞噬

中图分类号：R285.5 文献标识码：A 文章编号：1674-506X（2024）01-0088-0005

Study on Immune Regulation Function of Zebrafish Treated 

with Pogostemon Perilla Tea

XU Yiqiao¹, SONG Juanjuan^2,3*, ZHOU Bin^2,3, FENG Jie¹, LI Wei^2,3, WANG Chen^2,3, WANG Zhongwen^2,3, 

CAO Jing^2,3, HAO Jiangwei^2,3, HUANG Pan^2,3, FU Yali⁴

(1.Hunter Biotechnol Inc., Ltd., Hangzhou 310051,China; 2.Jing Brand Chizhengtang Pharmaceutical Co., Ltd., Huangshi 435000,China;

 3.Hubei key Laboratory of Quality and Safety of Traditional Chinese Medicine Health Food，Jing Brand Co.，Ltd.，Daye 435100,China;

4.Huangshi Food and Drug Inspection and Testing Center,Huangshi 435000,China)

Abstract: To study the regulatory effects of Pogostemon Perilla tea,a kind of anti-epidemic tea,on immune function of zebrafish with low immunity induced by vinorelbine tartrate.The zebrafish was intravenously injected with 0.2 mg/mL vinorelbine tartrate to induce hypoimmune model. According to random method,zebrafish were divided into normal control group,model control group,Pogostemon Perilla tea low-dose group(500 μg/mL),Pogostemon Perilla tea medium-dose group (1000 μg/mL),Pogostemon Perilla tea high-dose group(2000 μg/mL),30 tails per group.The effect of macrophage phagocytosis and T cell quantity in low immunity zebrafish was observed and analyzed by fluorescence microscopy. The experiment results showed that compared with model control group,the number of residual fluorescent microspheres in Pogostemon Perilla tea high-dose group (2000 μg/mL) was significantly lower than that in model control group (P<0.05). The fluorescence intensity of T cells in zebrafish in the low,medium,and high dose group of Pogostemon Perilla tea was significantly higher than that of model control group (P<0.01). Pogostemon Perilla tea has the effect of promoting macrophage phagocytosis in zebrafish and improving T cell reduction.

Keyword: Pogostemon Perilla tea; zebrafish; macrophage; T cells; phagocytosis

Doi: 10.3969/j.issn.1674-506X.2024.01-014

收稿日期：2023-03-03

基金项目：湖北中药质控标准物质研制及技术平台建立（2020ACA007）

作者简介：徐懿乔（1985-），女，硕士，高级工程师。研究方向：药理毒理研究。

*通信作者：宋娟娟（1986-），女，本科，工程师。研究方向：中医药及保健食品开发。

引用格式:徐懿乔,宋娟娟,周彬,等.基于斑马鱼模型的藿香紫苏饮免疫调节功能研究[J].食品与发酵科技,2024,60(1):88-92.