苏敏¹，吴彦蕾¹，邓惠玲²，董宪兵¹，陈 欣¹，黄大亮¹，周纯洁^1*

（1.重庆市食品药品检验检测研究院，国家市场监管重点实验室（调味品监管技术）

国家药品监督管理局麻醉精神药品质量监测重点实验室，重庆 401121；2.重庆市科学技术研究院，重庆401121）

摘要：为改进和优化鱼肉中多兽残检测的QuEChERS样品前处理方法，考察了提取剂、吸附剂以及浓缩过程对54种兽药提取回收率的影响，建立了以乙腈饱和的正己烷为分散剂，0.5%甲酸乙腈为提取剂，C18粉为吸附剂并采用稀释法减少溶剂效应的QuEChERS样品前处理方法，结合液相色谱-串联质谱（LC-MS/MS）技术可同时测定鱼肉中54种兽药残留。方法简便、快速、准确、可靠，适用于鱼肉样品中多兽药残留的快速检测。

关键词：QuEChERS；兽药残留；液相色谱-串联质谱；鱼肉

中图分类号：TS207.3   文献标识码：A   文章编号：1674-506X（2023）06-0135-0007

Rapid Determination of 54 Veterinary Drug Residues in Fish

by QuEChERS-LC-MS/MS

SU Min¹, WU Yanlei¹, DENG Huiling², DONG Xianbing¹, CHEN Xin¹

，HUANG Daliang¹, ZHOU Chunjie^1*

(1.Key Laboratory of Condiment Supervision Technology for State Market Regulation,NMPA Key Laboratory for

Quality Monitoring of Narcotic Drugs and Psychotropic Substances,Chongqing Institute for Food and Drug 

Control，Chongqing 401121，China；2.Chongqing Academy of Science and Technology，Chongqing 401121,China)

Abstract: In order to optimize and improve QuEChERS method for sample pretreatment of determination of multiple veterinary drug residues in fish，the effects of extractant，adsorbent and concentration process on the extraction efficiencies of the 54 veterinary drugs were investigated. The high performance liquid chromatographytandem mass spectrometry （LC-MS/MS） method for the simultaneous detection of the 54 veterinary drug residues was developed，using QuEChERS as the sample pretreatment method with the dispersant of acetonitrile saturated n- hexane， the extractant of 0.5% formic acid acetonitrile， the adsorbent of C18 powder， and the dilution operation to reduce solvent effects. This method is simple，rapid，accurate and reliable. It is suitable for the rapid detection of multiple veterinary drug residues in fish.

Keywords:QuEChERS;veterinary drug residues;high performance liquid chromatography-tandem mass spectrometry(LC-MS/MS); fish

doi：10.3969/j.issn.1674-506X.2023.06-023