矫艳平，李丽娜，陈雪娇，王小海，余 萍*

（江西仁仁健康微生态科技有限公司，樟树331200）

摘要：为提高玫瑰本草酵素在美容养颜方面的应用潜力，通过单因素试验和响应面试验优化其制备工艺以提高产物中多酚的含量，使用斑马鱼试验验证其美白、抗氧化功能。结果发现，玫瑰本草酵素最佳发酵条件为混合菌添加量8%、发酵温度35 ℃、发酵时间120 h，此时多酚含量为60.0 mg/g。3.91～15.6 μL/mL玫瑰本草酵素组均能极显著降低斑马鱼头部黑色素信号强度和活性氧（ROS）荧光值（P＜0.01），并随剂量的增加效果逐渐增强，且低剂量（3.91 μL/mL）的玫瑰本草酵素的抗氧化效果强于30.8 μg/mL谷胱甘肽。结果表明，优化后的玫瑰本草酵素具备较好的美容养颜功能。

关键词：玫瑰本草酵素；美容养颜；抗氧化；斑马鱼；响应面

中图分类号：Q939.9；TS205.5 文献标识码：A 文章编号：1674-506X（2025）03-0046-0007

Process Optimization of Rose Herbal Jiaosu and Analysis of Its Beautifying and Skin Care Functions

JIAO Yanping，LI Lina，CHEN Xuejiao，WANG Xiaohai，YU Ping*

(Jiangxi Renren Health Micro Ecological Technology Co., Ltd., Zhangshu 331200, China)

Abstract: In order to improve the application potential of rose herbal jiaosu in beautifying and skin care, the preparation process was optimized through single factor experiments and response surface experiments to increase the polyphenol content in the product, and its whitening and antioxidant functions were verified using zebrafish experiments. The results found that the optimal fermentation conditions for rose herbal jiaosu were 8% mixed bacteria addition amount, fermentation temperature 35 ℃, and fermentation time 120 h. At that time, the polyphenol content was 60.0 mg/g. All 3.91～15.6 μL/mL rose herbal jiaosu groups could significantly reduce the melanin signal intensity and reactive oxygen species (ROS) fluorescence value of zebrafish head (P＜0.01), and the effect gradually increased with the increase of dose, even at low doses (3.91 μL/mL), the antioxidant effect of rose herbal jiaosu was also stronger than that of 30.8 μg/mL glutathione. The results showed that the optimized rose herbal jiaosu had relatively good beautifying and skin care function.

Keywords: rose herbal jiaosu; beautifying and skin care; antioxidation; zebrafish; response surface

doi: 10.3969/j.issn.1674-506X.2025.03-007